Bioengineering Studies

Development of environment protection measures based on benthonic clay and sulfuric acid wastes

2025-02-08T20:40:32+01:00

The oil and gas industry causes man-made changes in all soil components, as well as in water and plants. Crude oil and gas are processed at the same time as the product is produced, and gas condensate and millions of tons of waste are ignored. Recycling of oil and gas waste products is essential. Sulfur is widely used in agriculture as a fertilizer and part to fight various plant pests. Soon, not only will the issue of using sulfur dioxide waste as raw materials be solved, but also the problem of contamination of environmental bodies with biological pollutants will be avoided. The use of sulfur-based waste from the oil industry is very effective for antibacterial formulations based on ammonium salts and natural bentonite materials. The developed antibacterial drug can be used to disinfect various facilities. The production tests conducted show the necessity of using the proposed disinfectants to improve the environment. This paste, which includes the proposed bactericidal preparation and bentonite clay in its composition, can be used in veterinary medicine to treat necrobacteriosis in animals. Based on our results, Ammonium sulfate and potassium sulfate obtained from sulfur-containing wastes can be used as fertilizers, and their mixture with bentonite clay can be used as an enhancer in agriculture.

Comparison of Sysmex DI-60 digital optical microscopy and manual optical microscopy in leukocyte differentiation

2025-03-04T21:43:14+01:00

Manual microscopic differentiation of leukocytes is the primary tool for the diagnosis and monitoring of various diseases. Recently, digital optical microscopy has become a more common method, being an alternative to the conventional one, and therefore, there is a need to investigate its compatibility in more detail. The objective is to compare the results of digital and manual microscopy in the analysis of different leukocyte types and to assess the linearity, systemic and proportional differences between the methods. 109 samples were analyzed by manual and digital microscopy (Sysmex DI-60), and the comparison of the obtained results was performed by Passing-Bablok and Bland-Altman analysis. The linearity of the methods is satisfactory in all cells, except basophils and blasts. A statistically significant systemic difference was detected in segmented neutrophils, immature granulocytes, and lymphocytes, and correlations between the methods range from very strong to moderate, depending on the type of cells. Significant deviations were observed in leukocytosis and leukopenia. The results of the conducted analysis indicate a good correlation between digital and manual microscopy, but the identified systemic and proportional differences indicate the significance of the reclassification offered by the analyzer. In cases of severe leukopenia and leukocytosis, it is recommended to use manual microscopy as an additional check.

Advancing medicine with biomaterials: Innovations in regenerative technologies and clinical applications

2025-04-29T09:36:55+02:00

Biomaterials, both natural and synthetic, play a crucial role in medical applications by interacting with biological systems to treat or replace tissues. These materials must exhibit biocompatibility to avoid complications like immunological rejection and be degradable to ensure proper breakdown within the body after fulfilling their intended function. Common natural biomaterials include collagen, gelatin, and alginates, while synthetic materials such as polyurethane, fibronectin, and ceramics are also widely used. Over the past decade, there has been significant progress in the field of biomaterials, driven by advances in regenerative medicine and tissue engineering. These materials are now frequently used in a variety of clinical applications, from tissue healing and molecular probes to nanoparticle biosensors and drug delivery systems. Despite the progress, understanding how biomaterials interact, integrate, and function in complex biological environments remains a significant challenge. The ability of biomaterials to restore and enhance biological functions, particularly in areas such as tissue engineering, orthopedic surgery, and neural implants, has demonstrated substantial improvements in patient outcomes and quality of life. Key to their success in these applications are their biocompatibility, long-term stability, and effective integration with host tissues. This paper explores the evolving role of biomaterials in medical practice, evaluating their potential, current use, and ongoing challenges in clinical settings, with a focus on their contributions to healthcare advancements and patient care.